Transcriptome profiling of 60 human lung cell lines by strand-specific RNA sequencing after rRNA depletion

We performed deep RNA sequencing of 60 human lung cell lines: 50 lung adenocarcinoma cell lines, 7 NSCLC (non small cell lung cancer, non-adenocarcinoma) cell lines, 3 non-transformed lung cell lines. Total RNA was isolated and subjected to rRNA depletion to maintain also non-polyadenylated transcripts. Strand-specific next generation sequencing (NGS) using Illumina HiSeq allowed us to distinguish reads in the sense and antisense direction. The cell lines were sequenced in 2 or 3 replicates. Reads were aligned to the human reference genome GRCh38 using STAR mapper. The expression of 58,096 genes was calculated in terms of FPKM for every sample. Gene expression profiling of NSCLC lung cancer cell line panel