Data on direct lysis microplate RT-qPCR assay optimisation and applications
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Fig1_RT.xlsxOptimisations of thermocycler time and reaction mix Fig2_WB.svgCorrelation of chemiluminescent western blot signal to cell lysate loading. Human fibroblasts were lysed and processed by enhanced chemiluminescent blot probed for GADD34 (left). Cellular protein visualized by stain-free fluorescent imaging (right). Fig3_Applications.xlsxApplication of DL-RT-qPCR microplate assay to charactersiation of neural cell cultures, astrocyte IL6 activation, fibroblast IFNB1 and GADD34 activation Fig4_Differentiations.xlsxApplication of DL-RT-qPCR microplate assay to optimisation of astrocyte differentiation conditions based on S100B and GFAP expression, and neuronal differentiations by S100B, NEFH, SYN1 and VGLUT1 expression DL-RT-qPCR Extended Data.xlsxExtended Data
