Transcriptomic profiling in NTAPP-exposed human dermal papilla cells.

To screen biological effects of NTAPP, we performed bulk RNA sequencing on NTAPP-treated human dermal papilla (hDP) cells. Pathway enrichment analysis revealed broad biological effects of NTAPP on hDP cells. Particulaarly, supervised gene ontology analysis suggested that NTAPP-treated hDP cells showed transcriptomic changes in genes associated with Wnt/beta-catenin signaling pathway. Total RNAs were directly purified and isolated from NTAPP-treated hDP cells or non-treated hDP cells.