Nanopore Transposon Insertion Identification
收藏资源简介:
Supplementary Information for a manuscript investigating the ability of using nanopore sequencing for identifying the sites of transposon insertions in bacterial genomes. <br>Includes raw .ab1 files for sequencing to verify some transposon insertions and documents describing these insertions, a document describing the creation of all strains sequenced within the manuscript, a gel picture of genomic DNA prior to sequencing using a Rapid Kit as well as genomic DNA prior to sequencing using the LSK109 kit, a fasta file containing query sequences for identifying transposon insertions in genomes, a file showing coverage intervals for reads mapped back to the DBL494 genome after CRISPR-Cas enrichment and sequencing, a perl program used to pull a subset of reads of interest from a fasta file with input from blast outfmt-6,



