Supporting data for "Chromosome-Level Genome of the Venomous Snail <i>Kalloconus canariensis</i>: A Valuable Model for Venomics and Comparative Genomics"

Genomes are powerful resources to understand the evolutionary mechanisms underpinning the origin and diversification of the venoms of cone snails (Conidae: Caenogastropoda), and could aid in the development of novel drugs. br /Here, we used PacBio CLR reads and Omni-C data to assemble the chromosome-level genome of iKalloconus canariensis/i, a vermivorous cone endemic to the Canary Islands. The final genome size was 2.87 Gb, with a N50 of 79.75 Mb and 91% of the reads located into the 35 largest scaffolds. Up to 49.55% of the genome was annotated as repetitive regions, being Class I of transposable elements (18.56%) predominant. The annotation estimated 34,241 gene models. Comparative analysis of this genome with the two cone snail genomes released to date (iDendroconus betulinus/i and iLautoconus ventricosus/i) revealed similar genome sizes and organization, although chromosome sizes tended to be shorter in iK. canariensis/i. Phylogenetic relationships within subclass Caenogastropoda were recovered with strong statistical support. The family Conidae was recovered as a clade, with iK. canariensis/i plus iL. ventricosus sister/i to iD. betulinus/i. br /Despite the great diversity of cone snails (900 species) and their venoms (hundreds of peptides per species), only two recently reported genomes are available for the group. The high-quality chromosome-level assembly of iK. canariensis/i will be a valuable reference for studying the origin and evolution of conotoxin genes as well as whole genome duplication (WGD) events during gastropod evolution.基因组是解析芋螺科（Conidae）：新腹足亚纲（Caenogastropoda）毒液起源与演化多样性背后进化机制的重要资源，同时可助力新型药物的研发。本研究利用PacBio CLR读段（PacBio CLR reads）与Omni-C数据（Omni-C data），组装得到了加那利芋螺（Kalloconus canariensis）的染色体水平基因组（chromosome-level genome），该物种为加那利群岛特有的食蠕虫性芋螺。最终组装的基因组大小为2.87 Gb，N50值为79.75 Mb，91%的测序读段可锚定至35个最大的支架序列（scaffold）。该基因组中高达49.55%的区域被注释为重复序列区，其中以I类转座元件（transposable elements）占比18.56%为绝对主导。基因注释共预测得到34241个基因模型。本研究将该基因组与目前已发表的两种芋螺基因组——波纹芋螺（Dendroconus betulinus）与宽口芋螺（Lautoconus ventricosus）——进行比较分析，结果显示三者基因组大小与结构均较为相似，但加那利芋螺（K. canariensis）的染色体长度相对更短。新腹足亚纲（Caenogastropoda）内的系统发育关系得到了较强的统计学支持。芋螺科（Conidae）被鉴定为一个单系支系，其中加那利芋螺（K. canariensis）与宽口芋螺（L. ventricosus）共同构成一支，与波纹芋螺（D. betulinus）互为姊妹群。 尽管芋螺类群现存约900个物种，且每个物种的毒液中含有数百种芋螺毒素肽，但目前该类群仅公开了两份基因组数据。本次组装获得的高质量染色体水平加那利芋螺（K. canariensis）基因组，将为研究芋螺毒素基因的起源与演化，以及腹足类演化过程中的全基因组复制（WGD）事件提供重要参考依据。

搜集汇总

背景与挑战

背景概述

该数据集为有毒蜗牛Kalloconus canariensis的染色体水平基因组提供支持数据，包括基因组组装结果（大小为2.87 Gb，N50为79.75 Mb）和基因注释（34,241个基因模型），以及来自毒腺和足肌肉组织的RNA和DNA样本数据。数据集旨在用于毒液组学和比较基因组学研究，帮助理解锥螺毒液的进化机制和全基因组复制事件。