RNA-seq of adrenal cortical cells of Cacna1h-deficient mice against wild-type controls

CaV3.2 (encoded by Cacna1h gene) is necessary for the generation of calcium oscillations by maintaining voltage oscillations in zona glomerulosa cells of mice. However, Cacna1h knockout mice have normal plasma aldosterone levels, suggesting additional calcium entry pathways. We used RNA-seq to investigate such pathways in the murine zona glomerulosa. We want to investigate whether other calcium channels or transporters were upregulated to compensate for the loss of CaV3.2.CaV3.2（由Cacna1h基因（Cacna1h gene）编码）是通过维持小鼠肾小球带（zona glomerulosa）细胞的电压振荡以产生钙振荡所必需的分子。然而，Cacna1h基因敲除小鼠的血浆醛固酮水平却表现正常，这提示体内存在额外的钙内流通路。我们借助RNA测序（RNA-seq）技术对小鼠肾小球带组织中的这类通路展开研究，旨在探究是否存在其他钙通道或转运蛋白被上调，以弥补CaV3.2的功能缺失。