Results in Ct of the SYBR Green real-time PCR with various primer concentrations.
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All plasmids, at 10−5 dilution each, were tested at Tm 45°C. ‘C’: number of plasmid copies use as template.
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2015-12-02
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All plasmids, at 10−5 dilution each, were tested at Tm 45°C. ‘C’: number of plasmid copies use as template.