INA of LX2/XVir

Glioblastomas (GBM) are difficult to treat and deadly brain tumors and may infiltrate the whole brain. Cancer killing (oncolytic) viruses have been used to treat GBMs. However, oncolytic virotherapy needs surgery as the viruses have to be injected directly into the tumor. We used human cells that we loaded with the oncolytic virus XVir-N-31. Virus-loaded cells were applied into the noses of GBM-mice by a non-surgical method, were rapidly transported towards the brain tumor and also to invaded GBM cells located far away from the original tumor. In the brain these shuttle cells released XVir-N-31 which then infects and kills the cancer cells. In consequence, mice that received XVir-N-31 loaded shuttle cells via the nose showed a delayed tumor growth and better survival. In addition, if the intranasal delivery was combined with an intratumoral injection of XVir-N-31, 25% of mice did not show any tumors and survived long time.胶质母细胞瘤（Glioblastomas, GBM）是一类难治且致死性的脑部肿瘤，可浸润整个脑组织。杀伤肿瘤的溶瘤（oncolytic）病毒已被应用于胶质母细胞瘤的治疗，但此类溶瘤病毒疗法需通过手术将病毒直接注射入肿瘤部位。本研究采用装载有溶瘤病毒XVir-N-31的人类细胞，通过非手术方式将其滴注至GBM模型小鼠的鼻腔。这些携带病毒的细胞可快速向脑内肿瘤部位迁移，同时也能抵达远离原发肿瘤的浸润性胶质母细胞瘤细胞。在脑内，这些穿梭细胞会释放XVir-N-31，进而感染并杀伤肿瘤细胞。实验结果表明，经鼻腔递送携带病毒的穿梭细胞的小鼠，肿瘤生长延迟且生存期更优。此外，若将鼻腔给药与瘤内注射XVir-N-31联合使用，25%的小鼠未检测到肿瘤，且实现了长期存活。