Profiling the proteomic inflammatory state of human astrocytes using DIA mass spectrometry

In the present study we used DIA MS to characterize the profile of over 4,000 proteins in immortalized human astrocytes exposed to TNF, IL-1β, and LPS, as well as in primary human astrocytes cocultured with brain endothelial cells and exposed to LPS. For both immortalized and primary astrocytes, DIA MS data were matched against a spectral library generated by merging DDA MS runs from both unfractionated and fractionated cell lysates.