HIGH-THROUGHPUT METHOD OF FOR MITOCHONDRIA ISOLATION FROM PLANT SEEDS

The invention relates to methods of extracting mitochondrial DNA from whole seeds in a high-throughput environment. The method comprises grinding a population of whole seeds， preferably wheat or barley seeds; isolating the mitochondria from the seeds; and extracting the mitochondrial DNA. Methods also relate to the use of low-speed centrifugation， which permits the methods use in a high-throughput environment. The invention relates to methods of extracting mitochondrial DNA from whole seeds in a high-throughput environment. The method comprises grinding a population of whole seeds， preferably wheat or barley seeds; isolating the mitochondria from the seeds; and extracting the mitochondrial DNA. Methods also relate to the use of low-speed centrifugation， which permits the methods use in a high-throughput environment.