List of proteins identified in 3 replicates of different digestion methods ( Urea, In-gel, FASP and RapiGest) for both CN (Centrigugation) and PN (Precipitation) samples for lysosome enriched samples
Mass spectrometry (MS)-based identification of ubiquitinated sites requires trypsin digestion prior to MS analysis, and a signature peptide was produced with a diglycine residue attached to the ubiqui
Robust, efficient, and reproducible protein extraction and sample processing is a key step for bottom-up proteomics analyses. While many sample preparation protocols for mass spectrometry have been de
