Here, we investigate how delays in plasma processing affect peptide-centric “bottom-up” proteomics, using multiple reaction monitoring mass spectrometry (MRM-MS). We used validated Tier 2-level assay
This study aims to assay self-cleaving activity of the generated glmS ribozymes by RfamGen. Cleavage reaction was induced by ligand addition at 10 mM for various incubation time. Cleaved and uncleaved
Stochasticity between independent LC–MS/MS runs is a challenging problem in the field of proteomics, resulting in significant missing values (i.e., abundance measurements) among observed peptides. To