Additional file 5: Table S1. of Increasing quality, throughput and speed of sample preparation for strand-specific messenger RNA sequencing

Alignment-based metrics for reverse transcriptase comparisons. Table S2. Alignment-based metrics for the size selection experiment. Table S3. Alignment-based metrics for the comparison of library construction kits. Table S4. Alignment-based metrics for the intermediate ssRNA-seq pipeline. The protocol evaluated includes all changes (1st strand cDNA synthesis, optimal bead purifications, new library construction chemistry with modified ligation condition, bead-based size selection, and UNG treatment) with the exception of the mRNA isolation improvements. Table S5. Alignment-based metrics for the final ssRNA-seq pipeline using UHR. Table S6. Alignment-based metrics for the final ssRNA-seq pipeline using tumor samples. (XLS 62Â kb)用于逆转录酶比较分析的基于比对的评估指标。表S2。用于片段筛选实验的基于比对的评估指标。表S3。用于文库构建试剂盒比较分析的基于比对的评估指标。表S4。用于中间阶段单链RNA测序（ssRNA-seq）流程的基于比对的评估指标。表S5。本研究所评估的实验方案涵盖全部优化改动，包括第一链cDNA合成、优化磁珠纯化工艺、采用改良连接条件的新型文库构建化学体系、基于磁珠的片段筛选以及尿嘧啶DNA糖苷酶（UNG）处理，但未包含mRNA分离环节的优化改进。用于使用UHR的最终单链RNA测序（ssRNA-seq）流程的基于比对的评估指标。表S6。用于使用肿瘤样本的最终单链RNA测序（ssRNA-seq）流程的基于比对的评估指标。（XLS 62 KB）