Global miRNA transcriptomic profiling of permanent focal ischemia in an in vivo rat model

Focal ischemia is triggered by the sudden significant reduction of blood supply to the brain, as a result of either the rupture or occlusion by thrombus/embolism of a blood vessel in the brain. Permanent focal ischemia occurred when blood supply to a specific part of the brain is impeded without reperfusion. Despite major steps achieved in the elucidation of the patho-physiology of cerebral ischemia, the available therapeutic avenues for acute ischemic stroke remain scarce. Cell cycle re-activation has been revealed as a novel signaling pathway during permanent focal ischemia. As such, non-specific aurora kinase inhibitor ZM447439, has been injected intracranial-ventricularly 30min post-ischemia induction to determine its efficacy in reduction of neuronal damage in terms of infarct volume.局灶性脑缺血（focal ischemia）由脑部血管因血栓（thrombus）或栓塞（embolism）发生破裂或闭塞，导致脑部血液供应突然大幅减少所诱发。当特定脑区的血液供应受阻且未实现再灌注时，便会引发永久性局灶性脑缺血。尽管目前在阐明脑缺血的病理生理学（patho-physiology）机制方面已取得诸多重要进展，但急性缺血性脑卒中的可用治疗途径依然匮乏。研究表明，细胞周期再激活是永久性局灶性脑缺血过程中的新型信号通路。据此，本研究于缺血诱导后30分钟通过脑室内注射非特异性极光激酶（aurora kinase）抑制剂ZM447439，以评估其在缩小梗死体积、减轻神经元损伤方面的疗效。