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Primer sequences used for cloning.
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2017-10-28
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(a) Primer list used to clone greater amberjack leptin; (b) Comparable sequence from closely related species for primer design; (c) Primers for greater amberjack real-time PCR.
(a) Primer list used to clone greater amberjack leptin; (b) Comparable sequence from closely related species for primer design; (c) Primers for greater amberjack real-time PCR.
NIAID Data Ecosystem50
Primers for 18S rRNA cloning and for real-time RT-PCR.
“Accession Number” refers to the accession for the sequence in NCBI (18S rRNA) or the UF ProbeName for the corresponding microarray probe. Transcripts: Ctr1c, copper transporter 1c; TepII, thiolester
NIAID Data Ecosystem40
Oligonucleotide primers used in the amplification of the protein coding sequences in this study.
The underlined nucleotides indicate overlapping base pairs with the generic primers for the ORFs cloned using the Gateway® system. The bold underlined nucleotides show the restriction site for the app
NIAID Data Ecosystem20
Additional file 7 of Evolution of the codling moth pheromone via an ancient gene duplication
Additional file 7: Table S5. Primer sequences used for amplification and cloning of desaturase genes.
Figshare2021-04-24 更新40
List of primers used for PCR-amplification of full-size genes encoding the studied proteins.
arestriction enzyme sites are underlined in the primer sequences. bto express hexokinase in Pichia pastoris, the forward primer with the BglII site instead of XhoI was used for PCR amplification of ge
NIAID Data Ecosystem20
