To identify the downstream target genes of PUS1 on breast cancer cells, we established MDA-MB-231 cell lines in which PUS1 has been knocked down by shRNA. We then performed gene expression profiling
SUM52PE cells were lentivirally transduced with PKLO empty vector control (Sigma SHC001 MISSION PLKO.1-PURO CONTROL VECTOR) or shRNA specific for C11orf67 (Sigma Clone ID: NM_024684.2-280s21c1 SHCLND-
To identify changes in splicing patterns following WTAP and TPR knockdown, RNA-sequencing was performed on breast cancer cells transfected with siRNAs targeting WTAP or TPR. Overall design: Libraries
