CRISPR knockout screens using Cas9 variants
收藏NIAID Data Ecosystem2026-05-01 收录
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To systematically evaluate the efficiency and off-target tolerance of Cas9 variants in complex with different single guide RNAs (sgRNAs), we applied high-throughput viability screens and a synthetic paired sgRNA- target system to assess thousands of sgRNAs in combination with two high-fidelity Cas9 variants HiFi and LZ3.
创建时间:
2023-05-30



