Extraction buffer omitting protease inhibitor was prepared and 1 protease inhibitor tablet was dissolved in 20ml. The extracts were prepared as follows: six flies expressing lacZ (DJ694,Bg2 recombinan
Insight into the molecular determinants of thermal stability in halohydrin dehalogenase HheD2. Descriptor: 2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID, GLYCEROL, Short-chain dehydrogenase/reductase SDR
Vela or Imig activities were assayed as described in Methods. The t½ was the time at which GCase activity decreased to 50% of initial values. n = 3 to 6 for all assays.
