Abcc6^em1Qlju

This allele was made by ZFN mutagenesis.ZFNs were designed to target exon 1 of rat Abcc6 gene at the binding site/cutting site 5-CACGCCTGGAGAGTCCTGcgcaggCCTGAGGGTGAGTCC-3 (c.24-c.62). The resulting mutation is a 10-bp deletion from cDNA position 44-53 (CAGGCCTGAG).本等位基因由ZFN诱变技术构建而成。ZFNs被设计用于靶向大鼠Abcc6基因的第1外显子，作用于结合/切割位点5-CACGCCTGGAGAGTCCTGcgcaggCCTGAGGGTGAGTCC-3（c.24-c.62）。由此产生的突变是在cDNA的第44-53位（CAGGCCTGAG）处发生了一个10碱基的缺失。