Gene expression profiles in E3.0 WT and Klf5 KO embryos

Klf5 has essential functions during early embryogenesis and in the derivation of ES cells from inner-cell mass of blastocyst. Among Kruppel-like factor (Klf) family members, only Klf5 shows peri-implantation lethal phenotype, but the precise mechanisms still remain unknown. To understand and identify molecular mechanisms, we performed microarray analysis by using E3.0 WT and Klf5 KO embryos when first phenotype of Klf5 deficiency appears. Wild type (WT) and Klf5 knock-out (KO) embryos were collected from oviduct at E3.0. cDNAs were synthesized from individual embryos in accordance with the protocol described by Kurimoto et al., 2007. Gene expression analysis was performed by GeneSpring GXsoftwareKlf5在早期胚胎发生以及从囊胚内细胞团（inner-cell mass）分离建系胚胎干细胞（ES cells）的过程中发挥关键必需功能。在Kruppel样因子（Kruppel-like factor, Klf）家族成员中，仅Klf5呈现着床前后致死表型，但其确切分子机制仍未阐明。为解析并鉴定相关分子机制，我们在Klf5缺陷首次出现表型的胚胎发育第3.0天（E3.0）阶段，利用野生型（wild type, WT）与Klf5敲除（knock-out, KO）胚胎开展了基因芯片分析（microarray analysis）。我们于胚胎发育第3.0天（E3.0）从输卵管中收集野生型（wild type, WT）与Klf5敲除（knock-out, KO）胚胎，按照Kurimoto等人2007年发表的实验方案，从单个胚胎中合成互补DNA（cDNAs），随后使用GeneSpring GX软件完成基因表达分析。