Genome_Wide_Subnuclear_Architecture_in_Antigenically_Selected_Lines_of_P_falciparum_. Genome_Wide_Subnuclear_Architecture_in_Antigenically_Selected_Lines_of_P_falciparum_

We have used chromatin conformation capture and its derivatives to study the nuclear architecture in lines of P.falciparum that express distinct variant antigens. Chemical crosslinking of cells followed by proximity based ligation and the subsequent enrichment of ligated junctions allows us to estimate contact probabilities for all genomic loci at a resolution of 5Kb - 50Kb. We evaluate these contact probabilities in the context of polymer folding models to deduce the scaling principles and folding architecture of interphase P. falciparum chromosomes. We consider evidence for spatial segregation of subtelomeric chromosomal territories involved in var gene expression as well as the anchoring of heterochromatic regions to the nuclear periphery. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/