mRNA-seq of peripheral blood monocyte- and human pluripotent stem cell-derived macrophages

We investigate the impact of an initial stimulation and a re-stimulation in altering transcriptional responses in both peripheral blood-monocyte derived macrophages and human pluripotent stem cell derived macrophages. Overall design: mRNA-seq in 2-3 replicates of LPS stimulated macrophages derived from peripheral blood or human pluripotent stem cell-derived macrophages