LC-MS/MS Analysis of affinity purified septins.
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A. nidulans wildtype (WT_No Tag) and septin S-tag strains were grown for 4–5 h (isotropic), 8–9 h (unicellular) and 16 h (multicellular) at 30°C in liquid media with shaking. Developmental stages were monitored by microscopy. Proteins were isolated, immunoprecipitated and analyzed by LC-MS/MS. The average of two biological replicates is shown.
创建时间：
2014-03-24
