Gene expression profiles of Hes1 positive retinal subsets

To analyze Mueller glia specific gene expression, Hes1-promoter-dEGFP mice was used. dEGFP positive and negative retinal fractions were purified by a cell sorter and subjected to RNA-seq Examination of mRNA expression patterns in Hes1-positive (Hes1P) retinal cells and Hes1-negative (Hes1N) retinal cells at 2 developmental timepoints.为分析米勒胶质细胞（Mueller glia）的特异性基因表达，本研究采用了Hes1启动子驱动dEGFP的转基因小鼠。通过细胞分选仪纯化得到dEGFP阳性与阴性的视网膜组分，并进行RNA测序（RNA-seq），以检测两个发育时间点下Hes1阳性（Hes1P）视网膜细胞与Hes1阴性（Hes1N）视网膜细胞的mRNA表达模式。