miR-seq analysis of nucleus pulposus tissues from compression-induced intervertebral disc degeneration rats

Intervertebral disc degeneration (IDD) is an important cause of low back pain. And abnormal mechanical factors are an important contributor to IDD. To identify mechanical responsive miRNAs in IDD, we used a custom-made compression device to establish IDD in rats and considered as IDD group, and the Sham group was inserted with K-wires into coccyxes only. After four weeks, rat nucleus pulposus tissues were obtained for miR-seq analysis.椎间盘退变（Intervertebral disc degeneration, IDD）是引发下腰痛的重要病因。异常力学因素是导致IDD的重要诱因。为识别IDD中机械响应的miRNA，本研究采用定制化加压装置构建大鼠椎间盘退变模型（设为IDD组），假手术组仅向大鼠尾椎置入克氏针。饲养4周后，采集大鼠髓核组织进行miR-seq测序分析。