Single-site CpG Profiling for KMT2D and HNF4A genomic region in pancreatic cancer cells.

Differential CpG methylation profiling for KMT2D and HNF4A genomic region in pancreatic cancer cells was performed using Illumina MiSeq. Triplicates of 5-AZA-CdR treated or untreated cells were used for genomic DNA isolation and subjected to Targeted 5-mC Bisulfite Sequencing for the selected regions of interest i) chr12: 49448986-49449286 for KMT2D and ii) chr20: 43029895, chr20: 42984320, chr20: 42984324, chr20: 43029732, chr20: 43036036, chr20: 42984099, chr20: 42984209, chr20: 42984394, chr20: 42984878, chr20: 42984338, chr20: 42984579, chr20: 42984347, chr20: 42984453, chr20: 42983920, chr20: 42984276, chr20: 43029777 for HNF4A. Primers used were designed and validated by Zymo Research Corporation.