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Peptidome analysis of the hypothalamus in a single mouse.

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NIAID Data Ecosystem2026-03-13 收录
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A 2-mg frozen section of hypothalamus from a single mouse was homogenized in 200 µL of denaturing solution(7 M urea, 2 M thiourea, 20 mM dithiothreitol) containing cOmplete Mini Protease Inhibitor Cocktail (Roche). Peptide extracts equivalent to 135 µg of tissue were analyzed by LC-MS/MS.

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2022-01-08
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