The effects of UVB and the organic osmolyte betaine on rat epidermal keratinocytes in an organotypic culture system

The expression of several genes relevant to epidermal biology, proliferation/differentiation or malignancy as well as solute transport were verified by independent methods (qRT-PCR, western blotting). The data concerning changes in calcium metabolism after UVB exposure has been published separately (Bart et al., Br. J. Dermatol. 171:376-387, 2014).本研究采用实时荧光定量逆转录聚合酶链式反应（qRT-PCR）与蛋白质印迹法（Western blotting）两种独立实验方法，验证了多个与表皮生物学、细胞增殖/分化、恶性病变及溶质转运相关的基因的表达水平。有关紫外线B（Ultraviolet B，简称UVB）照射后钙代谢变化的相关数据已单独发表（Bart等，《英国皮肤病学杂志》，171卷：376-387，2014年）。