Transcription profiling of mouse Notch1 deficient in hair follicles

Notch1 deficient hair matrix keratinocytes have lower mitotic rates, resulting in smaller follicles with fewer cells. In addition, the ratio of melanocytes to keratinocytes is greatly reduced. Microarray was performed to study downstream mechanism of Notch1-deficiency Experiment Overall Design: Microarray was first performed using HF_emN1-1 and HF_anN1-1 samples. Later, HF_emN1-2, HF_anN1-2, HF_WT-1, and HF_WT-2 were used to perform another set of microarray. anN1s and WTs were used as wild-type controls.Notch1缺陷型毛囊基质角质形成细胞（hair matrix keratinocytes）的有丝分裂速率更低，进而形成细胞数目更少、体积更小的毛囊。此外，黑素细胞（melanocytes）与角质形成细胞（keratinocytes）的比例大幅降低。本研究通过基因芯片（microarray）技术探究Notch1缺陷的下游调控机制。实验整体设计：首先使用HF_emN1-1与HF_anN1-1样本完成首轮基因芯片实验；后续又采用HF_emN1-2、HF_anN1-2、HF_WT-1及HF_WT-2样本开展了另一组基因芯片实验，其中anN1样本与WT样本均作为野生型对照。