The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA), then these libraries were sequenced on the Illumina sequencing platform (HiSeqTM 2500 or Ill
We collected female and male Glossina fuscipes fuscipes from the field (Uganda) and determined the Spiroplasma infections status of each individual. We used RNA-seq to investigate the effects of Spiro