RNAseq of bone marrow stromal/stem cells under metabolic stress.

To obtain a global profile of gene expression, we performed RNA sequencing (RNA-seq) using cells cultured under different conditions to determine genes and signaling pathways associated with enhanced osteogenesis and cell survival in hBMSCs Three replicates were analyzed for three different patient groups The hBMSCs were established from bone marrow samples from 7 patients (mean age: 70 years, range: 50-90 years). A statement of consent was informed to all donors. All experiments were performed according to the relevant guidelines and regulations