Gene expression profiling of WT and Aldh1a1 KO mouse lung tissues

To explore potential impact of Aldh1a1 deficiency in the retinoic acid signaling in the lung, gene expression levels were compared between wildtype and Aldh1a1 deficient lung tissues. The expression levels of retinoic acid responsive genes were very similar between wildtype and Aldh1a1 deficient lungs, suggesting that Aldh1a1 deficiency has minimal impact on retinoic acid signaling. Lung tissues were isolated from two mice per group (Aldh1a1+/+ and Aldh1a1-/-) and enzymatically digested. Extracted and purified total RNA samples were used for RNA-seq analysis.为探究醛脱氢酶1A1（Aldh1a1）基因缺失对肺组织内视黄酸信号通路（retinoic acid signaling）的潜在影响，本研究比较了野生型（wildtype）与Aldh1a1缺失型肺组织的基因表达水平。视黄酸应答基因（retinoic acid responsive genes）的表达水平在野生型与Aldh1a1缺失型肺组织中高度相似，提示Aldh1a1基因缺失对视黄酸信号通路的影响微乎其微。本研究每组各取2只小鼠的肺组织（Aldh1a1+/+与Aldh1a1-/-），经酶解消化后提取并纯化总RNA，所得样品用于RNA测序（RNA-seq）分析。