Transcriptome annotation

Assembled transcripts and predicted peptides of <i>L. chagosensis</i><i> </i>were searched against the GenBank non-redundant sequence, UniProtKB/Swiss-Prot, and human proteome v.11.5 from STRING v.11 databases with an e-value cut-off of 1x10^-5. Protein domains were identified by mapping the peptide sequences against Pfam 32.0 database using HMMER v3.3. The top Blastx and Blastp hits in UniProtKB/Swiss-Prot database and Pfam annotations for each transcript were then used as input into Trinotate v3.2.2 (Haas 2015) to generate a comprehensive annotation report. <br>针对查戈斯岛藻（*L. chagosensis*）的组装转录本与预测肽段，以E值阈值（e-value cut-off）1×10^-5为筛选标准，分别比对至GenBank非冗余序列数据库（GenBank non-redundant sequence）、UniProtKB/Swiss-Prot数据库（UniProtKB/Swiss-Prot），以及STRING v.11数据库中的人类蛋白质组v.11.5数据集。 通过HMMER v3.3工具将肽段序列比对至Pfam 32.0数据库，完成蛋白质结构域的鉴定。 随后将UniProtKB/Swiss-Prot数据库中Blastx与Blastp的最优匹配结果，以及各转录本的Pfam注释信息作为输入，导入Trinotate v3.2.2工具（Haas，2015）以生成综合注释报告。