Spatial transcriptomics of 3-month-old and 18-month-old mouse ovarian and uterine tissues

We used Visium technology (10X Genomics) to infer cell-to-cell communication in ovarian and uterine tissue based on spatial proximity. Organs from 3-month mice in diestrus and 18-month old mice were collected and frozen in OCT. 10 µm thick tissue slices were placed on Visium Spatial Gene Expression Slides (10X Genomics) and stained with Hematoxylin and Eosin (H&E). Libraries were prepared by manufacturer's recommendations and sequenced on NovaSeq6000. For samples that were sequenced in two runs, both sequencing runs were merged when running spaceranger (10X Genomics). Original nd2 microscopy images and results of scRNA-seq (linked datasets) and spatial transcriptomics analysis are available at Biostudies (S-BIAD482 and S-BSST852).本研究依托10X Genomics公司的Visium技术，基于空间邻近性特征推断卵巢与子宫组织的细胞间通讯关系。收集动情间期3月龄小鼠与18月龄小鼠的器官组织，采用OCT包埋后冷冻保存。将厚度为10 μm的组织切片置于Visium空间基因表达玻片（10X Genomics）上，随后进行苏木精-伊红（Hematoxylin and Eosin，H&E）染色。按照制造商推荐的实验流程构建测序文库，并在NovaSeq6000测序平台上完成测序。对于分两轮测序的样本，在运行10X Genomics的spaceranger分析软件时，将两轮测序数据进行合并处理。原始nd2格式显微镜图像、单细胞RNA测序（scRNA-seq，关联数据集）结果及空间转录组分析数据，均可在Biostudies数据库获取，对应登录号为S-BIAD482与S-BSST852。