ONT-RCA error profiler: benchmarking Nanopore sequencing errors in RCA-amplified circular genomes

This dataset and code repository accompany the manuscript “Read-Level Error Characterization of Long-Read Nanopore-Based Sequencing of the Circular DNA Virome” submitted to Nucleic Acids Research (June 2026). It contains the full reproducible pipeline (ONT-RCA error profiler) used to quantify Nanopore sequencing error rates in RCA-amplified circular DNA, along with representative data subsets, example configuration files, and scripts for generating figures and summary tables. The code implements error extraction via CIGAR parsing and depth profiling across controlled M13mp18 dilution series and Anellovirus-positive plasma samples.Contents:- ONT-RCA-error-profiler_v1.0 (code, environment file, README). All code is mirrored at GitHub: https://github.com/MERIDAIN-Lab/ONT-RCA-error-profiler- Example_Data.tar.gz (sample data and summary tables) - Supplementary_Figures