Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (RNA-Seq I). Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (RNA-Seq I)

Vascular endothelial cells play a pivotal role in whole-body homeostasis. To test the function of human long non-coding RNA LEENE in Human umbilical vein endothelial cells (HUVEC), we inhibited LEENE by using a previously validated locked nucleic acid (LNA) GapmeR under pulsatile flow which mimics the physiological state and is characterized by elevated LEENE levels. PolyA-enriched RNA-seq was performed to examine the LEENE-regulated gene expression. Overall design: PolyA-enriched RNA-seq in HUVEC with LEENE knockdown by LNA