Analysis of variance of relative <i>Ldh</i> expression in 18 clones of <i>Daphnia pulex, D. pulicaria</i> and hybrids.

The number of Ldh transcripts per Gapdh transcript was calculated using the 2−ΔCT method and is the dependent variable in this analysis. Gene is LdhA or LdhB; Group refers to D. pulex, D. pulicaria, and D. pulex-pulicaria hybrids; temperature is 10°C or 20°C; dissolved oxygen is high (6.5–7 mg/l) or low (2–3 mg/l). Interactions are indicated by an asterisk (*). Sources of variation that show a significant difference in gene expression (p<0.05) are in bold-face type.