The isolated tumor tissues and cancer cells were washed twice with precooled normal saline. To remove proteins and extract metabolites, the tissues were smashed on ice, suspended in 400 μL of precoole
LC-ESI-MS analysis of the Z. lotus fruit extract and literature review of the DPPH• radical-scavenging presented as extract concentration needed to scavenge 50% of DPPH• (IC50) values.
