Constitutive heterochromatin controls nuclear mechanics, morphology, and integrity through H3K9me3 mediated chromocenter compaction, Manning et al raw data

<b>Figure 1. Heterochromatin histone modification states can be modulated distinctly.</b><b>Figure 2. Nuclear blebbing and rupture increase upon loss of H3K9me3 while loss of H3K9me2 decreases this effect.</b><b>Figure 3. Nuclear blebbing and rupture upon loss of H3K9me3 is due to interphase-based behaviors and not dependent on mitotic failures.</b><b>Figure 4. Nuclear stiffness increases upon loss of H3K9me2 and decreases upon loss of H3K9me3.</b><b>Figure 5. Chromocenter compaction increases upon loss of H3K9me2, decreases upon loss of H3K9me3, and is unchanged by loss of H3K27me3.</b><b>Supplemental Figure 1. Phospho-MLC2, lamin A/C, and HP1α levels remain unchanged by inhibitors altering histone modifications.</b><b>Supplemental Figure 2. H3K9me3 chromocenter heatmaps, DNA compaction, and nucleus characteristics.</b><b>Supplemental Figure 3. Nuclear blebbing and chromocenter compaction are restored upon dual knockdown of H3K9me2 and H3K27me3.</b>