This is from the transposon mutant library of Pseudomonas aeruginosa PAO1. The aim is to check for the presence of PL sub complexes in the absence of FliF protein.
These are H_pylori cells that are lacking FliP protein. In these cells, FliF protein was knocked out . to examine its relation to the hat-like complex.
AD1231: pAD1361/A136; Carbenicillin 50; virB, virD4, virGN54D on multicopy plasmid, host lacks a Ti-plasmid. Constitutive virG mutant, does not require AS. But good to have AS in the media, anyway.