MOESM2 of Functional analysis of granulocyte and monocyte subpopulations in neonates
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Additional file 2: Figure S1. A/B Gating strategy for granulocyte and monocyte subpopulations and their activation marker: Representative probe of a new-born infant for activation marker on granulocyte and monocyte subpopulation. After single cell gating and determination of living cells by ZOMBIE, cells were gated by SSC-A and subpopulation marker (CD14, CD16, CD62L) in their subpopulation according to FMOs. CD14dim monocytes and CD16dim neutrophil population was distinguished by gating the 25th percentile of main neutrophil population.
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2019-11-30
