Identification of genes associated with TGF-beta/Smad3-mediated renal inflammation and fibrosis by RNA sequencing

In this study, we employed high-throughput RNA sequencing (RNA-Seq) to identify the Smad3-dependent lncRNAs related to renal inflammation and fibrosis in Smad3 knockout (KO) mouse models of unilateral ureteral obstructive nephropathy (UUO) and immunologically-induced anti-glomerular basement membrane glomerulonephritis (anti-GBM GN). 12 kidney tissue samples of Smad3 KO/WT mice from normal control, UUO at day 5 or anti-GBM GN at day 10 models (n=2 in each group) for whole transcriptome RNA-sequencing.本研究采用高通量RNA测序（RNA-Seq）技术，在单侧输尿管梗阻性肾病（UUO）及免疫诱导型抗肾小球基底膜肾小球肾炎（anti-GBM GN）的Smad3基因敲除（KO）小鼠模型中，筛选与肾脏炎症及纤维化相关的Smad3依赖型长链非编码RNA（lncRNAs）。我们收集了正常对照、造模第5天UUO模型及造模第10天anti-GBM GN模型的Smad3敲除（KO）/野生型（WT）小鼠肾脏组织样本共12份（每组n=2），用于全转录组RNA测序。