Germinal centre-driven maturation of B cell response to SARS-CoV-2 mRNA vaccination

We used ultrasound-guided fine needle aspiration of the draining ipsilateral axillary lymph nodes to interrogate SARS-CoV-2 mRNA vaccine-induced GC responses in humans. Examination of 56 single-cell RNA-seq samples from PBMC and lymph node taken at multiple time points post-vaccination across multiple participants. Data processing steps: 1) Single cell feature counts for a single library were generated using `cellranger count` from Cell Ranger v6.0.1. 2) Single cell feature counts for multiple libraries were then pooled by subsampling to the same effective sequencing depth using `cellranger aggr`. Genome build: GRCh38 Processed data files format and content: 1) matrix.mtx.gz contains a gene expression count matrix stored in the Market Exchange Format for sparse matrices 2) features.tsv.gz contains feature information that corresponds to the row index of matrix.mtx.gz 3) barcodes.tsv.gz contains cellular barcodes that correspond to column indices of matrix.mtx.gz. The trailing dash and number (e.g. -12) indicate the sample from which the cell originated and correspond to the row number for that sample in aggregation.csv. 4) aggregation.csv contains sample information. The order of the samples represented as rows in aggregation.csv corresponds to the numbers after the dash (e.g. -12) in barcodes.tsv.gz.