Hg-MATE-Db.v1.01142021

<b>Hg-MATE-Db: Hg-</b>cycling<b> M</b>icroorganisms<b> </b>in<b> A</b>quatic<b> </b>and<b> T</b>errestrial<b> E</b>cosystems<b> D</b>ata<b>b</b>ase <br>Microorganisms play a significant role in regulating the form and fate of mercury (Hg) in aquatic and terrestrial ecosystems. Microbes with the <i>hgcAB</i> gene pair can produce a more toxic, and bioaccumulative form of Hg, methylmercury (MeHg). Microbes that possess the <i>mer</i> operon can demethylate and/or reduce Hg species as part of a detoxification mechanism. Improved techniques for capturing <i>hgcAB</i> and <i>mer </i>presence and diversity are necessary for identifying the major microbial players in environmental Hg cycling. The primary goal of the Hg-MATE-Db is to provide an up-to-date collated resource of Hg-cycling genes from pure culture and environmental microbial genomes and meta-omic datasets. The database will be updated regularly.<br><br>The Hg-MATE-Db.v1 contains an<b> </b><i>hgcAB </i>dataset with resources for identifying key microbial producers of the toxin MeHg. Future versions of the Hg-MATE-Db<b> </b>will also include <i>hgcAB</i> sequences from high-throughput sequencing and clone datasets. Future versions will also contain a <i>mer </i>dataset, which will contain resources for identifying genes of the <i>mer-</i>operon that encode for demethylation of organomercurials (<i>merB</i>), reduction of inorganic Hg(II) (<i>merA</i>), as well as operon regulation (<i>merR</i>), and Hg transport across the cell (<i>merTPC</i>).<br>The latest release, version 1 (v1), was compiled on 23 October 2020 and finalized on 14 January 2021, and contains an <i>hgcAB </i>dataset. The catalogue contains 1053 unique HgcA/B amino acid sequences. <br><br> Please cite us when using the Hg-MATE-Db in your work. Gionfriddo, C., Capo, E., Peterson, B., Lin, H., Jones, D., Bravo, AG., Bertilsson, S., Moreau, J., McMahon, K., Elias, D., and Gilmour, C. (2021). Hg-MATE-Db.v1.01142021. doi:10.25573/serc.13105370

**Hg-MATE-Db：水生与陆地生态系统中汞循环微生物数据库**

微生物在调控水生与陆地生态系统中汞（Hg）的形态与归趋方面发挥着关键作用。携带<i>hgcAB</i>（hgcAB）基因对的微生物可合成毒性更强、生物累积性更高的汞形态——甲基汞（methylmercury，MeHg）。携带<i>mer</i>操纵子（mer operon）的微生物则可通过脱甲基化与/或还原汞物种的方式实现解毒机制。当前亟需优化用于检测<i>hgcAB</i>与<i>mer</i>的存在情况及多样性的技术，以明确环境汞循环中的核心微生物类群。Hg-MATE-Db的核心目标是提供一份最新整合的资源库，收录纯培养菌株及环境微生物基因组与宏组学数据中的汞循环基因，且该数据库将定期更新。

Hg-MATE-Db.v1 包含<i>hgcAB</i>数据集，可用于识别产毒甲基汞的关键微生物类群。未来版本的Hg-MATE-Db还将纳入高通量测序与克隆文库数据集来源的<i>hgcAB</i>序列，同时新增<i>mer</i>数据集：该数据集将涵盖用于识别<i>mer</i>操纵子相关基因的资源，包括编码有机汞脱甲基化功能的<i>merB</i>、无机汞(II)还原功能的<i>merA</i>、操纵子调控功能的<i>merR</i>，以及跨细胞汞转运功能的<i>merTPC</i>。

最新正式版本为v1，于2020年10月23日完成汇编，2021年1月14日定稿，仅包含<i>hgcAB</i>数据集。该目录共收录1053条独特的HgcA/B氨基酸序列。

若您的研究工作中使用了Hg-MATE-Db，请引用如下文献：Gionfriddo, C., Capo, E., Peterson, B., Lin, H., Jones, D., Bravo, AG., Bertilsson, S., Moreau, J., McMahon, K., Elias, D., and Gilmour, C. (2021). Hg-MATE-Db.v1.01142021. doi:10.25573/serc.13105370

搜集汇总

背景与挑战

背景概述

Hg-MATE-Db.v1.01142021是一个专注于汞循环微生物基因的数据库，当前版本包含1053个独特的HgcA/B氨基酸序列，主要用于识别环境中产生甲基汞的关键微生物。数据库将定期更新，未来计划扩展至mer操纵子等更多基因资源。