Cells (2×10 5 ) were cultured in 6-well plates, and then were incubated with sunitinib for 24 hr. Necrosis was analyzed using the Annexin V-FITC/PI Apoptosis Detection Kit (40302ES50, Yeasen
Representative images in the revised S2C Fig were generated from raw files woGF_4_ch1/2 (W/O GF); EF10cyc_4_ch1/2 (E+F10+Cyc); EF1shh_7_ch1/2 (E+F1+Shh). (ZIP)
The in situ detection of caspase-3 activity has applications in the imaging and monitoring of multiple pathologies, notably cancer. A series of cell penetrating FRET-based fluorogenic substrates were