Host Factor Experiment (ICL010-R)

Purpose: To obtain samples from Calu-3 cells infected with A/Netherlands/602/2009 (H1N1) and A/CA/04/09 (H1N1) for transcriptional analysis and proteomics analysis for A/Netherlands/602/2009. For A/Netherlands/602/2009 and mock infected the time points are 0, 3, 7, 12, 18, 24, 30, 36 and 48 h post infection. For A/CA/04/09 the time points are 0,12, 24 and 48 h post infection.There are triplicate samples for all conditions for both transcriptional and proteomics analyses. Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates; Time matched mocks done in triplicate from same cell stock as rest of samples. Inoculation medium for mock infection was the same as the medium used for virus infection. Infection done at an MOI of 3