Precise modulation of genome functions and live tracking of genome regions in CRISPR/dCas9 knockin mice. Precise modulation of genome functions and live tracking of genome regions in CRISPR/dCas9 knockin mice

we demonstrated ex vivo and in vivo validation of dCas9 KI functions such as transcription Overall design: live mRNA profiles of wild type (WT) and SunTag-dCas9 mice were generated by deep sequencing.Myod1 actived and control live mRNA profiles of SunTag-dCas9 mice were generated by deep sequencing.