Additional file 1: of Ovarian cancer stem cells and macrophages reciprocally interact through the WNT pathway to promote pro-tumoral and malignant phenotypes in 3D engineered microenvironments

Figure S1.â U937 monocytes differentiated in 3D hanging drop arrays are equivalent to U937 monocytes differentiated in 2D. Figure S2. No change in proliferation in CSC compartments of hetero-spheroids. Figure S3. Gating strategy for Flow cytometry. Figure S4. Cancer cells do not significantly express the macrophage marker, CD206. Figure S5 CD163 expression is elevated in CSC/U937â M2 hetero-spheroids. Figure S6. Macrophages do not significantly express elevated ALDH. Figure S7. phospho-STAT3 is significantly reduced in CSC/shWNT5B-M2 hetero-spheroids compared to CSC/M2 hetero-spheroids. Figure S8. Kuramochi-CSC also drive elevated CD206 expression in macrophages, and polarized macrophages enrich ALDH+ cells in Kuramochi CSC and resistance to carboplatin. Figure S9. High-grade serous ovarian cancer Patient 259 derived CSC drive elevated CD206 expression in macrophages, and demonstrate a carboplatin resistant phenotype. Figure S10. Scatter plots for correlation of WNT5B with immune cell subsets in ovarian carcinoma. Table S1. List of primers used for qPCR experiments. (ZIP 1916 kb)