Effects of DNA-demethylation on the expression of plastic and transgenerational responses to nitrogen availability in annual plant species

The dataset contains plant trait information of 6 annual species (grasses and non legume-forbs).  A parental generation was raised under N (nitrogen) limitation and balanced nutrient supply, from which an offspring generation was grown under either the same or another option of these nutrient conditions. Further, offspring plants were subjected to a demethylation treatment, with the use of the agent Zebularine (Z). This treatment comprised a control (Z0µM) or Z50µM. Plants of both the parental and offspring generation were raised in a greenhouse. Plants of the offspring generation were harvested after five weeks and traits were measured. , , # Effects of DNA-demethylation on the expression of plastic and transgenerational responses to nitrogen availability in annual plant species Dataset DOI: [10.5061/dryad.bzkh189rj](https://doi.org/10.5061/dryad.bzkh189rj) ## Description of the data and file structure Dataset DOI: [10.5061/dryad.bzkh189rj](https://doi.org/10.5061/dryad.bzkh189rj) This dataset relates to the article “Effects of DNA-demethylation on the expression of plastic and transgenerational responses to nitrogen availability in annual plant species” published in Evolutionary Ecology (doi: 10.1007/s10682-025-10374-z), by Vanessa Minden, Conchita Alonso, Harry Olde Venterink and Koen J.F. Verhoeven. Corresponding author Vanessa Minden, email: [[e-mail]](mailto:[e-mail]) The dataset contains plant trait information of 6 annual species (grasses and non legume-forbs). A parental generation was raised under N (nitrogen) limitation and balanced nutrient supply, from which an offspring genera..., ,