This is the teaching material for the Drosophila module of the Developmental Genetics RSM (research skills module) held at the Faculty of Life Sciences at The University of Manchester in 2014
RNAseq of sea lily Metacrinus rotundus was conducted using Illumina Hiseq2000 platform. Seqenced RNA were extracted from several larval stages, including bipinnaria larvae (61 hpf), early doriolaria l
Comparisons were carried out using developing seeds harvested at 10 days after pollination. Fold change was calculated dividing the expression in the mutant by the expression in the wild-type.